ANA tests for SLE Lupus
This part of the ANA test gives an estimate of how many anti-nuclear antibodies are present (i.e. the “titre”).

This is done by taking a sample of the liquid part of the blood called the serum and diluting it with a liquid called a diluent. The process is like making orange juice from a frozen concentrate. One can is diluted into 4 cans of water (the diluent).

For the ANA test, 1 part of the serum is diluted into 40 parts of diluent (called a “1:40” titre). This diluted sample is tested for the presence of ANA antibodies. If the test is positive, a new sample is made at half the strength (1:80) and tested for the presence of ANA. The test is repeated every time it is positive by diluting the sample to (Phone number can only be seen by the question and answer creators), 1:1280:]], and finally 1:2560.

The lowest dilution is 1:2560. When antibodies are present at the lowest dilution, this indicates that there is a very high number of antibodies in the blood. In other words, the body has mounted a substantial immune response against nuclear proteins.

The ANA test is a test of “autoimmunity.” Autoimmune means that the body has failed to recognize its own parts as self, which allows the immune system to mount a response against the body’s own cells and tissues. Systemic Lupus Erythematosus (SLE) is an example of an autoimmune disease. In people with SLE, the body mounts an immune response to specific proteins found inside the nucleus of their cells.

Why the ANA test is done
The ANA test is a simple screening test to look for antibodies in the blood that are attacking proteins in the nucleus. A limitation of the ANA test is that it does not identify which protein the antibodies are attacking in the nucleus. It just indicates that there are antibodies in the blood against proteins in the nucleus.

So sorry for your loss - sending healing thoughts and hugs
And hoping for best possible outcome re auto immune tests
I have serone negativd Rheumotoid arthritis auto immune disorder
but tested negative in blood results diagnosed through symptoms but the meds are really helping me

The ANA blood test detects the auto-antibodies present in an individual's blood serum. The common tests used for detecting and quantifying ANAs are indirect immunofluorescence and enzyme-linked immunosorbent assay (ELISA). In immunofluorescence, the level of auto-antibodies is reported as a titre. This is the highest dilution of the serum at which auto-antibodies are still detectable. Positive auto-antibody titres at a dilution equal to or greater than 1:160 are usually considered as clinically significant. Positive titres of less than 1:160 are present in up to 20% of the healthy population, especially the elderly. Although positive titres of 1:160 or higher are strongly associated with autoimmune disorders, they are also found in 5% of healthy individuals. Auto-antibody screening is useful in the diagnosis of autoimmune disorders and monitoring levels helps to predict the progression of disease.
Such as Fibromyalgia or Lupus.

💜⭐💜⭐💜⭐💜

@A MyFibroTeam Member. Sorry for your loss. I was tested for Lupus via ANA test and mine return speckled everytime. They have no answers. Going to a Lupus clinic on the 28th of this month. Hopefully they will have some answers

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